LOCUS       sequence-58012-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Azpiazu I, Gautam N
  TITLE     A fluorescence resonance energy transfer-based sensor indicates that
            receptor access to a G protein is unrestricted in a living mammalian
            cell.
  JOURNAL   J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12.
  PUBMED    15078878
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-06-25"; volume: "279(26)"; pages: "27709-18. 
            Epub 2004 Apr 12"
COMMENT     Sequence Label: alpha-0 CFP in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(461..489)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccactag tccagtgtgg tggaattcat gggatgtact ctgagcgcag aggagagagc
      181 cgccctcgag cggagcaagg cgattgagaa aaatctcaaa gaagatggca tcagcgccgc
      241 caaagacgtg aaattactcc tgctgggggc tggagaatca ggaaaaagca ccattgtgaa
      301 gcagatgaag atcatccatg aagatggctt ctctggagaa gacgtaaagc agtacaagcc
      361 tgtcgtctac agcaacacca tccagtctct ggcagccatt gtgcgggcca tggatactct
      421 gggcggtacc atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      481 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      541 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      601 gccaacactt gtcactactc tcacttgggg tgttcaatgc ttttcaagat acccagatca
      661 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      721 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      781 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      841 tggacacaaa ttggaataca actatatctc acacaatgta tacatcacgg cagacaaaca
      901 aaagaatgga atccaaagct a
//
