LOCUS       sequence-58014-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKIP-hph
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(200..304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     390..406
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     391..407
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    429..462
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(561..584)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
       61 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      301 cgcgcacatt tccccgaaaa gtgccacctt agggataaca gggtaattac ggccccaagg
      361 tccaaacggt gaaggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
      421 cgaattccga agttcctatt ctctagaaag tataggaact tcaggtctga agaggagttt
      481 acgtccagcc aagctagctt ggctgcaggt cgtcgaaatt ctaccgggta ggggaggcgc
      541 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
      601 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
      661 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
      721 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
      781 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
      841 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
      901 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
//
