LOCUS       sequence-58019-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKRED
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..73
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt gggaattcga
       61 gctctaagga ggttataaaa aatgcatcaa aaaaaccagg taatgaacct gggtccgaac
      121 tctaaactgc tgaaagaata caaatcccag ctgatcgaac tgaacatcga acagttcgaa
      181 gcaggtatcg gtctgatcct gggtgatgct tacatccgtt ctcgtgatga aggtaaaacc
      241 tactgtatgc agttcgagtg gaaaaacaaa gcatacatgg accacgtatg tctgctgtac
      301 gatcagtggg tactgtcccc gccgcacaaa aaacaacgtg ttaaccacct gggtaacctg
      361 gtaatcacct ggggcgccca gactttcaaa caccaagctt tcaacaaact ggctaacctg
      421 ttcatcgtta acaacaaaaa aaccatcccg aacaacctgg ttgaaaacta cctgaccccg
      481 atgtctctgg catactggtt catggatgat ggtggtaaat gggattacaa caaaaactct
      541 accaacaaat cgatcgtact gaacacccag tctttcactt tcgaagaagt agaatacctg
      601 gttaagggtc tgcgtaacaa attccaactg aactgttacg taaaaatcaa caaaaacaaa
      661 ccgatcatct acatcgattc tatgtcttac ctgatcttct acaacctgat caaaccgtac
      721 ctgatcccgc agatgatgta caaactgccg aacactatct cctccgaaac tttcctgaaa
      781 taagcatgcc cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac
      841 cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccg
//
