LOCUS       sequence-58047-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Moss FJ, Imoukhuede PI, Scott K, Hu J, Jankowsky JL, Quick MW, 
            Lester HA
  TITLE     GABA transporter function, oligomerization state, and anchoring: 
            correlates with subcellularly resolved FRET.
  JOURNAL   J Gen Physiol. 2009 Dec;134(6):489-521.
  PUBMED    19948998
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2009-12"; volume: "134(6)"; pages: "489-521"
COMMENT     Sequence Label: pcDNA3.1(+)mGAT1-577-CFP-578CT
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(128..838,839..841,842..844))
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(170..191)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     519..538
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     778..799
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgggccct ctagactcga gcggccgcca ctgtgctgga tatctgcaga attcgctaga
       61 tgtaggcctc cttgctggct gagctgccag cctgcggctg ctcagggcca ttctcagggc
      121 gcacaatctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      181 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca
      241 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      301 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga
      361 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgatgtag ttgtactcca
      421 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      481 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      541 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      601 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccccaggtc agggtggtca
      661 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      721 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
      781 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
      841 ccatatcttc actgggctga atcatgacct ggagacgctg cttcagggag cccttcaggg
      901 tgaggaacat g
//
