LOCUS       sequence-58063-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Straathof KC, Pule MA, Yotnda P, Dotti G, Vanin EF, Brenner MK, 
            Heslop HE, Spencer DM, Rooney CM
  TITLE     An inducible caspase 9 safety switch for T-cell therapy.
  JOURNAL   Blood. 2005 Jun 1. 105(11):4247-54.
  PUBMED    15728125
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2005
            Jun 1. 105(11):4247-54."
COMMENT     Sequence Label: pMSCV-F-del Casp9.IRES.GFP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..370
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(722..733)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttacgccacc atgctcgagg gagtgcaggt
       61 ggagactatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt
      121 gcactacacc gggatgcttg aagatggaaa gaaagttgat tcctcccggg acagaaacaa
      181 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc
      241 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc
      301 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct
      361 aaaactggaa tctggcggtg gatccggagt cgacggattt ggtgatgtcg gtgctcttga
      421 gagtttgagg ggaaatgcag atttggctta catcctgagc atggagccct gtggccactg
      481 cctcattatc aacaatgtga acttctgccg tgagtccggg ctccgcaccc gcactggctc
      541 caacatcgac tgtgagaagt tgcggcgtcg cttctcctcg ctgcatttca tggtggaggt
      601 gaagggcgac ctgactgcca agaaaatggt gctggctttg ctggagctgg cgcggcagga
      661 ccacggtgct ctggactgct gcgtggtggt cattctctct cacggctgtc aggccagcca
      721 cctgcagttc ccaggggctg tctacggcac agatggatgc cctgtgtcgg tcgagaagat
      781 tgtgaacatc ttcaatggga ccagctgccc cagcctggga gggaagccca agctcttttt
      841 catccaggcc tgtggtgggg agcagaaaga ccatgggttt gaggtggcct ccac
//
