LOCUS       sequence-58073-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Wang S, Zhao Y, Leiby M, Zhu J
  TITLE     A new positive/negative selection scheme for precise BAC 
            recombineering.
  JOURNAL   Mol Biotechnol. 2009 May;42(1):110-6. doi: 
            10.1007/s12033-009-9142-3. Epub 2009 Jan 22.
  PUBMED    19160076
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12033-009-9142-3"; journalName: "Mol Biotechnol"; date: 
            "2009-05"; volume: "42"; issue: "1"; pages: "110-6"
COMMENT     Sequence Label: pSK+KanaRpsL
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(830..849)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gaggtcgacg gtatcgataa gcttcacgct gccgcaagca ctcagggcgc aagggctgct
       61 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
      121 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
      181 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
      241 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
      301 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctaaccag gagctattta
      361 atggcaacag ttaaccagct ggtacgcaaa ccacgtgctc gcaaagttgc gaaaagcaac
      421 gtgcctgcgc tggaagcatg cccgcaaaaa cgtggcgtat gtactcgtgt atatactacc
      481 actcctaaaa aaccgaactc cgcgctgcgt aaagtatgcc gtgttcgtct gactaacggt
      541 ttcgaagtga cttcctacat cggtggtgaa ggtcacaacc tgcaggagca ctccgtgatc
      601 ctgatccgtg gcggtcgtgt taaagacctc ccgggtgttc gttaccacac cgtacgtggt
      661 gcgcttgact gctccggcgt taaagaccgt aagcaggctc gttccaagta tggcgtgaag
      721 cgtcctaagg cttaatggta gatctgatca agagacagga tgacggtcgt ttcgcatgct
      781 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      841 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgt
//
