LOCUS       sequence-58078-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH002
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(200..304)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             447..491
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             507..530
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             546..569
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
       61 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      301 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
      361 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcccctca gcgcggccgc
      421 tagcggatcc ttaaaaaacc atggttaaag aaacagcagc agcgaaattt gaaagacaac
      481 atatggatag tggaggcggt ggaagtttgg aagttctttt ccaaggaccg ggcggaggag
      541 gctcttggtc gcatccacag ttcgaaaaag ccatggccgg cggtggtgga tcgggaggag
      601 gaggttcggg tggcggagga tcgggcgcct caaaaggaga agaaacgaca atgggtgtta
      661 tcaagccaga catgaagatc aagcttaaaa tggagggtaa tgttaatggt cacgccttcg
      721 tcattgaggg agagggtgaa ggaaagccat acgatggaac taatactatt aacttggaag
      781 tgaaggaggg tgccccactt ccattctctt atgatatcct tactacagcc ttcgcatatg
      841 gaaatcgagc attcacaaaa taccca
//
