LOCUS       sequence-58079-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH009
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             439..483
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             499..522
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             538..561
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcccct cagcgcggcc gctagcggat
      421 ccttaaaaaa ccatggttaa agaaacagca gcagcgaaat ttgaaagaca acatatggat
      481 agtggaggcg gtggaagttt ggaagttctt ttccaaggac cgggcggagg aggctcttgg
      541 tcgcatccac agttcgaaaa agccatggcc gcctcaaaag gagaagaaac gacaatgggt
      601 gttatcaagc cagacatgaa gatcaagctt aaaatggagg gtaatgttaa tggtcacgcc
      661 ttcgtcattg agggagaggg tgaaggaaag ccatacgatg gaactaatac tattaacttg
      721 gaagtgaagg agggtgcccc acttccattc tcttatgata tccttactac agccttcgca
      781 tatggaaatc gagcattcac aaaataccca gacgacattc caaattactt caagcagtca
      841 tttccagagg gatattcttg ggagagaaca atgacattcg aggacaaggg tatagttaaa
      901 gtgaaatctg ata
//
