LOCUS       sequence-58081-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH013
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcccct cagcgcggcc gctagcggat
      421 ccttaaaaaa ccatggccgc ctcaaaagga gaagaaacga caatgggtgt tatcaagcca
      481 gacatgaaga tcaagcttaa aatggagggt aatgttaatg gtcacgcctt cgtcattgag
      541 ggagagggtg aaggaaagcc atacgatgga actaatacta ttaacttgga agtgaaggag
      601 ggtgccccac ttccattctc ttatgatatc cttactacag ccttcgcata tggaaatcga
      661 gcattcacaa aatacccaga cgacattcca aattacttca agcagtcatt tccagaggga
      721 tattcttggg agagaacaat gacattcgag gacaagggta tagttaaagt gaaatctgat
      781 attagtatgg aagaagattc attcatctat gagatacacc tgaagggcga gaactttcct
      841 ccgaatggac cagtgatgca gaaaaagaca acaggatggg acgca
//
