LOCUS       sequence-58082-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH026
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..79)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(95..118)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(134..157)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gccctcagcg cggccgcggt acctattaag cccatgagtc catatgctgt ctctcgaatt
       61 ttgcagccgc tgtctcttta ctaccacctc ctccaggtcc ttggaagaga acttccaatg
      121 atcctccacc tcctttctca aattgtggat gtgaccatcg cgaacctcct cctcccgatc
      181 cgcctcctcc actaccacca cctccggata cgtagagctc gtccatacca tcagttgagt
      241 ttctagctac tgctgactca tacacagtca ctttattgta atctttatca tggttcaaaa
      301 tttcaattcg gtgatccaca aagtgatagt ctggcaattt cacagctttc ttagctctgt
      361 agattgtctt aaagtccact cgatgatgtc cacctccttc caacaacagt ttatgcttca
      421 cgtcaccttt caaaactcca tctctgacat acatacgttc agtacttgcg tcccatcctg
      481 ttgtcttttt ctgcatcact ggtccattcg gaggaaagtt ctcgcccttc aggtgtatct
      541 catagatgaa tgaatcttct tccatactaa tatcagattt cactttaact atacccttgt
      601 cctcgaatgt cattgttctc tcccaagaat atccctctgg aaatgactgc ttgaagtaat
      661 ttggaatgtc gtctgggtat tttgtgaatg ctcgatttcc atatgcgaag gctgtagtaa
      721 ggatatcata agagaatgga agtggggcac cctccttcac ttccaagtta atagtattag
      781 ttccatcgta tggctttcct tcaccctctc cctcaatgac gaaggcgtga ccattaacat
      841 taccctccat tttaagcttg atcttcatgt ctggcttgat aacacccatt gtcgtttctt
      901 ctccttttga ggcggccatg
//
