LOCUS       sequence-58083-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH030
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..55)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(65..85)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccctcagcg cggccgcggt acctattaag cgctaacttt tcgcttcttc tttggtccac
       61 ctccgacttt tctctttttc tttggcgaac ctcctcctcc cgatccgcct cctccactac
      121 caccacctcc ggatacgtag agctcgtcca taccatcagt tgagtttcta gctactgctg
      181 actcatacac agtcacttta ttgtaatctt tatcatggtt caaaatttca attcggtgat
      241 ccacaaagtg atagtctggc aatttcacag ctttcttagc tctgtagatt gtcttaaagt
      301 ccactcgatg atgtccacct ccttccaaca acagtttatg cttcacgtca cctttcaaaa
      361 ctccatctct gacatacata cgttcagtac ttgcgtccca tcctgttgtc tttttctgca
      421 tcactggtcc attcggagga aagttctcgc ccttcaggtg tatctcatag atgaatgaat
      481 cttcttccat actaatatca gatttcactt taactatacc cttgtcctcg aatgtcattg
      541 ttctctccca agaatatccc tctggaaatg actgcttgaa gtaatttgga atgtcgtctg
      601 ggtattttgt gaatgctcga tttccatatg cgaaggctgt agtaaggata tcataagaga
      661 atggaagtgg ggcaccctcc ttcacttcca agttaatagt attagttcca tcgtatggct
      721 ttccttcacc ctctccctca atgacgaagg cgtgaccatt aacattaccc tccattttaa
      781 gcttgatctt catgtctggc ttgataacac ccattgtcgt ttcttctcct tttgaggcgg
      841 ccatggtttt ttaaggatcc gctagcggcc gcgctgaggg gctggcaagt gtagcggtca
      901 cgctgcgcgt aaccaccaca
//
