LOCUS       sequence-58084-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH078
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(196..300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
       61 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      301 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
      361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc ccctcagcgc ggccgctagc
      421 ggatccttaa aaaaccatgg ccgcctcaaa aggagaagaa acgacaatgg gtgttatcaa
      481 gccagacatg aagatcaagc ttaaaatgga gggtaatgtt aatggtcacg ccttcgtcat
      541 tgagggagag ggtgaaggaa agccatacga tggaactaat actattaact tggaagtgaa
      601 ggagggtgcc ccacttccat tctcttatga tgtaagttta aacagatcca tactaactaa
      661 cttgttctga cataattttc agatccttac tacagccttc gcatatggaa atcgagcatt
      721 cacaaaatac ccagacgaca ttccaaatta cttcaagcag tcatttccag agggatattc
      781 ttgggagaga acaatgacat tcgaggacaa gggtatagtt aaagtgaaat ctgatattag
      841 tatggaagaa gattcattca tctatgagat acacctgaag ggcgagaact ttcctccgaa
      901 tggaccagtg atgcagaaaa agacaacagg atgggacgca gactgactat
//
