LOCUS       sequence-58085-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH082
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      361 tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg ctagcggatc
      421 cttaaaaaac catggccgcc tcaaaaggag aagaaacgac aatgggtgtt atcaagccag
      481 acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc gtcattgagg
      541 gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa gtgaaggagg
      601 gtgccccact tccattctct tatgatgtaa gtttaaacag atccatacta actaacttgt
      661 tctgacataa ttttcagatc cttactacag ccttcgcata tggaaatcga gcattcacaa
      721 aatacccaga cgacattcca aattacttca agcagtcatt tccagaggga tattcttggg
      781 agagaacaat gacattcgag gacaagggta tagttaaagt gaaatctgat attagtatgg
      841 aagaagattc attcatctat gagatacacc tgaagggcga gaactttcct ccgaatggac
      901 ca
//
