LOCUS       sequence-58098-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength GCaMP3-based calcium indicator with improved 
            response. Please also see the GCaMP6s/m/f indicators..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS, Mutlu S, 
            Calderon NC, Esposti F, Borghuis BG, Sun XR, Gordus A, Orger MB, 
            Portugues R, Engert F, Macklin JJ, Filosa A, Aggarwal A, Kerr RA, 
            Takagi R, Kracun S, Shigetomi E, Khakh BS, Baier H, Lagnado L, Wang 
            SS, Bargmann CI, Kimmel BE, Jayaraman V, Svoboda K, Kim DS, 
            Schreiter ER, Looger LL
  TITLE     Optimization of a GCaMP calcium indicator for neural activity 
            imaging.
  JOURNAL   J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 
            10.1523/JNEUROSCI.2601-12.2012.
  PUBMED    23035093
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2601-12"; journalName: "J Neurosci"; date: 
            "2012-10-3- 3"; volume: "32"; issue: "40"; pages: "13819-40"
COMMENT     Sequence Label: pCMV-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             187..243
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     456..477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(583..604)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(844..863)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgccacca tgggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac catggtcgac
      181 tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc
      241 tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
      301 aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
      361 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc
      421 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      481 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
      541 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      601 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
      661 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      721 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      781 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
      841 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      901 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      961 ctggagtaca acctgccgga cca
//
