LOCUS       sequence-58133-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH058
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(129..233)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             376..420
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             436..459
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             475..498
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
       61 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      241 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      301 gttacgcgca gcgtgaccgc tacacttgcc agcccctcag cgcggccgct agcggatcct
      361 taaaaaacca tggttaaaga aacagcagca gcgaaatttg aaagacaaca tatggatagt
      421 ggaggcggtg gaagtttgga agttcttttc caaggaccgg gcggaggagg ctcttggtcg
      481 catccacagt tcgaaaaagc catggccggc ggtggtggat cgggaggagg aggttcgggt
      541 ggcggaggat cgggcgcctc aaaaggagaa gaaacgacaa tgggtgttat caagccagac
      601 atgaagatca agcttaaaat ggagggtaat gttaatggtc acgccttcgt cattgaggga
      661 gagggtgaag gaaagccata cgatggaact aatactatta acttggaagt gaaggagggt
      721 gccccacttc cattctctta tgatatcctt actacagcc
//
