LOCUS       sequence-58136-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH040
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(121..225)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(388..416)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
       61 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
      121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
      181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      241 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      301 cagcgtgacc gctacacttg ccagcccctc agcgcggccg ctagcggatc cttaaaaatg
      361 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      421 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      481 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
      541 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
      601 tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc
      661 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      721 aactacaaga cacgtaagtt taaac
//
