LOCUS       sequence-58140-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH042
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(168..272)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
       61 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      361 acacttgcca gcccctcagc gcggccgcta gcggatcctt aaaaatggtc tcaaagggtg
      421 aagaagataa catggcaatt attaaagagt ttatgcgttt caaggtgcat atggagggat
      481 ctgtcaatgg gcatgagttt gaaattgaag gtgaaggaga aggccgacca tatgagggaa
      541 cacaaaccgc aaaactaaag gtaagtttaa acatatatat actaactaac cctgattatt
      601 taaattttca ggtaactaaa ggcggaccat taccattcgc ctgggacatc ctctctccac
      661 agttcatgta tggaagtaaa gcttatgtta aacatccggc agatatacca gattatttga
      721 aactttc
//
