LOCUS       sequence-58144-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH090
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..64)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(80..103)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cgaattttgc agccgctgtc tctttactac cacctcctcc aggtccttgg aagagaactt
       61 ccaatgatcc tccacctcct ttctcaaatt gtggatgtga ccatcgcgaa cctcctcctc
      121 ccgatccgcc tcctccacta ccaccacctc cggatacgta gagctcgtcc ataccatcag
      181 ttgagtttct agctactgct gactcataca cagtcacttt attgtaatct ttatcatggt
      241 tcaaaatttc aattcggtga tccacaaagt gatagtctgg caatttcaca gctttcttag
      301 ctctgtagat tgtcttaaag tccactcgat gatgtccacc tccttccaac aacagtttat
      361 gcttcacgtc acctttcaaa actccatctc tgacatacat acgttcagta cttgcgtccc
      421 atcctgttgt ctttttctgc atcactggtc cattcggagg aaagttctcg cccttcaggt
      481 gtatctcata gatgaatgaa tcttcttcca tactaatatc agatttcact ttaactatac
      541 ccttgtcctc gaatgtcatt gttctctccc aagaatatcc ctctggaaat gactgcttga
      601 agtaatttgg aatgtcgtct gggtattttg tgaatgctcg atttccatat gcgaaggctg
      661 tagtaaggat atcataagag aatggaagtg gggcaccctc cttcacttcc aagttaatag
      721 tattagttcc atcgtatggc tttccttcac cctctccctc aatgacgaag gcgtgaccat
      781 taacattacc ctccatttta a
//
