LOCUS       sequence-58146-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5b
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             386..691
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     570..589
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gaattacaac agtactgcga tgagtggcag ggggggcgta aacgccgcgt ggatccggct
       61 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
      121 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
      181 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
      241 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
      301 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
      361 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
      421 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
      481 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
      541 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
      601 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
      661 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      721 tgcaggtcga tacagtagaa attacagaaa ctttatcacg tttagtaagt atagaggctg
      781 aaaatccaga tgaagccgaa cgacttgtaa gagaaaagta taagagttgt gaaattgttc
      841 ttgatgcaga tgattttcag gactatgaca ctagcgta
//
