LOCUS       sequence-58169-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-empty chr I
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..98)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..99)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      101..348
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(117..135)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(385..403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(424..440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(437..459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    448..464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(472..478,479..496,497..502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    517..538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(655..672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actaggctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca
      121 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
      181 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
      241 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
      301 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccgtcgaggg
      361 ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
      421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      781 ccagcaaaag gccagg
//
