LOCUS       sequence-58175-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of VMA1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-VMA1 chr I
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagcag gcttccagct
      121 gaccaccatg gctggtgcaa ttgaaaacgc tcgtaaggaa ataaaaagaa tctcattaga
      181 agaccatgct gaatctgaat atggtgccat ctattctgtc tctggtccgg tcgtcattgc
      241 tgaaaatatg attggttgtg ccatgtacga attggtcaag gtcggtcacg ataacctggt
      301 gggtgaagtc attagaattg acggtgacaa ggccaccatc caagtttacg aagaaactgc
      361 aggccttacg gtcggtgacc ctgtttcgag aacaggtaag cctctgtcgg tagaattggg
      421 tcctggtctg atggaaacca tttacgatgg tattcaaaga cctttgaagg ccattaagga
      481 agaatcgcaa tcgatttata tcccaagagg tattgacact ccagctttgg ataggactat
      541 caagtggcaa tttactccgg gaaagtttca agtcggcgat catatttccg gtggtgatat
      601 ttacggttcc gtttttgaga attcgctaat ttcaagccat aagattcttt tgccaccaag
      661 atcaagaggt acaatcactt ggattgctcc agctggtgag tacactttgg atgagaagat
      721 tttggaagtt gaatttgatg gcaagaagtc tgatttcact ctttaccata cttggcctgt
      781 tcgtgttcca agaccagtta ctgaaaagtt atctgctgac tatcctttgt taacaggtca
      841 aagagttttg gatgc
//
