LOCUS       sequence-58176-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of AVT1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-AVT1 chr I
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagcag gctccaccat
      121 gcctgagcaa gaaccattga gtcccaatgg ccgtaagcgc tctgaggttc actatatctc
      181 aatcccattg aacagaggtt ccgcattctc accagatgat tctgtatcac agtttcagtc
      241 tgacgggttc atgacacgta ggcaatccat attggaccat cctgtgggct catttaaggg
      301 agttaattct ttaagtcgat ttgcgacttc gttgaggaga gccaattcgt ttcgtaatat
      361 cgagctaaat gcggataatg aaagatcctt tttcaaagag agtaacgatg aaacctacga
      421 tccggatact ttagcaccag ctttggacgg tagaagatta tcagtaactt taaataatgc
      481 tggtcgcccg cgcattacta atttggctaa caacgataga gttagtacag ccagcatggc
      541 tattcacgat gatgattatg ggtccatcca aaattcaaca attggggatt ctgggtcaat
      601 attacgccct actgcctcct taacagaaat gatgagtggt ggagcgggga gaaggtttac
      661 aaataatgac atggattcaa ttgtagtgaa gagagtggag ggtgtagatg gtaaggtagt
      721 aactcttctt gctggtcagt cgaccgctcc acagaccata ttcaactcaa tcaatgtact
      781 gattggtatt gggctcctgg cgttacctct tggtttgaaa tacgccggct gggttatagg
      841 acttactatg cttgcaatct tcgccttagc gacatttt
//
