LOCUS       sequence-58178-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pKT127-SEP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..753
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(67..95)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     690..710
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggtcgacgga tcggtgacgg tgctggttta attaacatga gtaaaggaga agaacttttc
       61 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      121 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      181 actactggaa aactacctgt tccttggcca acacttgtca ctactttaac ttatggtgtt
      241 caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
      301 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      361 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      421 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taacgatcac
      481 caggtgtaca tcatggcaga caaacaaaag aatggaatca aagctaactt caaaattaga
      541 cacaacattg aagatggagg cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      601 ggcgatgggc ccgtcctttt accagacaac cattacctgt ttacaacttc tactctttcg
      661 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      721 attacacatg gcatggatga actatacaaa taaggcgcgc cacttctaaa taagcgaatt
      781 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatac
//
