LOCUS       sequence-58179-e         379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J
  TITLE     RNA-programmed genome editing in human cells.
  JOURNAL   elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
  PUBMED    23386978
REFERENCE   2  (bases 1 to 379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "elife."; 
            date: "2013"; volume: "2:e00471. doi"; pages: " 10.7554/eLife.00471.
            Epub 2013 Jan 29"
COMMENT     Sequence Label: pMJ920
FEATURES             Location/Qualifiers
     source          1..379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..79)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(86..106)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(119..145)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(125..151)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctcctcgcc cttgctcacg gatccgctgc tgccgttgct cccactacca atgccgtcca
       61 ctttccgctt tttcttagga tcttccacct tgcgtttttt cttgggggag ccgctgccca
      121 ggctggcata atcgggcacg tcatagggat aggcgtcgcc tcccagctga gacaggtcga
      181 tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc agcacctctt
      241 tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag gcggcagggg
      301 ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct ctgataggct
      361 tgtctctgtg cttgttgta
//
