LOCUS       sequence-58192-e        4113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4113)
  TITLE     Lok lab Strongyloides stercoralis plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPV476
FEATURES             Location/Qualifiers
     source          1..4113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(88..211)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(244..272)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1020..1040
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1686..1781)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1818..1837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1819..1837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1842..1858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1842..1858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1960..1979)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1971..2780
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2038..2057)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2927..3515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3416..3435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3669..3686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3845..3872)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3964..4050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4099..4113,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4113,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga attctacagg
       61 tcactaatac catctaagta gttggttcat agtgactgca tatgttgtgt tttacagtat
      121 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      181 gtttctcgtt caactttctt gtacaaagtg gccatgagta aaggagaaga acttttcact
      241 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      301 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      361 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
      421 tatttaaatt ttcagccaac acttgtcact actctcactt atggtgttca atgcttctcg
      481 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      541 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
      601 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
      661 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      721 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      781 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct
      841 gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      901 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      961 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1021 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggacga actatacaaa
     1081 tgataactag gaatttattt tgataagtct cgcgtatttt atttgtaata atctttttat
     1141 aagatcaatt tacatttata ctgataaaat tgacacaatt acacttttta ttataaaata
     1201 ttttaaaata tttctatcaa aaggtttatt tttgttttaa atataatggt tttttttgta
     1261 aaaaatactt atttcgtttg ataatcttta ttcagagatt tgaataattt taaataaagt
     1321 agtttagttt tcatataaaa gttaatggta tatataaata tttaaatagt aaagattgaa
     1381 tcagtattca aaatgacacc taaaagttat tctatttcta gctataacaa taataataaa
     1441 gataattatg atacagattt agaaaaaaaa ataaggttaa attaattaat tctattttta
     1501 attaaaatga tttaagggag ttaaatgaaa cagaaacaga ttgggtctct atatatttag
     1561 tttatgtaat gttatttttt ggtggaatac aaataacggt atattttact tcaatgtggc
     1621 catacttatc aacaataaat agtaaggcaa atgtagatac tttagggtgg gttgtagcac
     1681 tagtgcaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg
     1741 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt
     1801 aacgcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1861 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1921 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1981 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     2041 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     2101 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     2161 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     2221 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     2281 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     2341 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     2401 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     2461 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     2521 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     2581 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     2641 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     2701 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     2761 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     2821 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     2881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3061 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3301 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3781 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3841 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3901 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3961 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4021 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     4081 cgctagcatg gatgttttcc cagtcacgac gtt
//
