LOCUS       sequence-58193-e        3417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  TITLE     Lok lab Strongyloides stercoralis plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPV477
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    99..198
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(231..259)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1007..1027
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1072..1171)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1188..1207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1189..1207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1212..1228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1212..1228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1330..1349)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1341..2150
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1408..1427)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2297..2885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2786..2805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3039..3056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3215..3242)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(3334..3417,1..3))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
      121 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
      181 tgtacaaaaa agcaggctcc atgagtaaag gagaagaact tttcactgga gttgtcccaa
      241 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      301 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      361 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
      421 agccaacact tgtcactact ctcacttatg gtgttcaatg cttctcgaga tacccagatc
      481 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      541 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta
      601 actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg
      661 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      721 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      781 gaatcaaagt tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
      841 agaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      901 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      961 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1021 ttgtaacagc tgctgggatt acacatggca tggacgaact atacaaatga gacccagctt
     1081 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     1141 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     1201 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1261 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1321 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1381 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1441 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1501 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1561 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1621 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     1681 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1741 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1801 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1861 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     1921 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1981 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2041 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2101 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2161 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2221 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3001 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3061 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3121 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3181 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3241 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3301 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3361 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttat
//
