LOCUS       sequence-58206-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKIP-cat
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..83
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             102..761
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(168..187)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    792..825
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca tcggcatagt
       61 atatcggcat agtataatac gacaaggtga ggaactaaac catggagaaa aaaatcactg
      121 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      181 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      241 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      301 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      361 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      421 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      481 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      541 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
      601 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
      661 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
      721 aattacaaca gtactgcgat gagtggcagg gcggggcgtg aggggatcaa ttctctagag
      781 ctcgctgatc agaagttcct attctctaga aagtatagga acttcgatgg cgcctcatcc
      841 ctgaagc
//
