LOCUS       sequence-58207-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKIP-neo
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..84
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(166..185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcttcctca tctccgggcc tttcgacctg cagcctgttg acaattaatc atcggcatag
       61 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgggatc ggccattgaa
      121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
      301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat
      661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatca
//
