LOCUS       sequence-58216-e       12789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 12789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: SOX2 TALE Repressor (KRAB 1-75)
FEATURES             Location/Qualifiers
     source          1..12789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3859
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3850
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3807..3827
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6416..6436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6476..6670
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             6686..6739
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6740..6742,6743..7456)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6740..6742,6743..7453)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6782..6803)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7043..7062)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7390..7411
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7481..8069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7534..7554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7952..7963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8072..8088)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8073..8089)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8594..8774
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8800..8817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8806..9030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9076..9504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9163..9182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9373..9394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9513..9533)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9518..9847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9698..9833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9760..9779
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9895..9942
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9961..10335
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10465..10586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10502..10521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10556..10575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10635..10651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10635..10651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10648..10670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10659..10675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10683..10689,10690..10707,10708..10713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10728..10749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10866..10883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11037..11625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11117..11136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11796..12587,12588..12656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12419..12438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12657..12761)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
     3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3841 tccatttcag gtgtcgtgac gtacggccac catgtcgcgg acccggctcc cttccccacc
     3901 cgcacccagc ccagcgtttt cggccgactc gttctcagac ctgcttaggc agttcgaccc
     3961 ctcactgttt aacacatcgt tgttcgactc ccttcctccg tttggggcgc accatacgga
     4021 ggcggccacc ggggagtggg atgaggtgca gtcgggattg agagctgcgg atgcaccacc
     4081 cccaaccatg cgggtggccg tcaccgctgc ccgaccgccg agggcgaagc ccgcaccaag
     4141 gcggagggca gcgcaaccgt ccgacgcaag ccccgcagcg caagtagatt tgagaacttt
     4201 gggatattca cagcagcagc aggaaaagat caagcccaaa gtgaggtcga cagtcgcgca
     4261 gcatcacgaa gcgctggtgg gtcatgggtt tacacatgcc cacatcgtag ccttgtcgca
     4321 gcaccctgca gcccttggca cggtcgccgt caagtaccag gacatgattg cggcgttgcc
     4381 ggaagccaca catgaggcga tcgtcggtgt ggggaaacag tggagcggag cccgagcgct
     4441 tgaggccctg ttgacggtcg cgggagagct gagagggcct ccccttcagc tggacacggg
     4501 ccagttgctg aagatcgcga agcggggagg agtcacggcg gtcgaggcgg tgcacgcgtg
     4561 gcgcaatgcg ctcacgggag cacccctcaa cctgacccca gagcaggtcg tggccattgc
     4621 ctcgaatgga gggggcaaac aggcgttgga aaccgtacaa cgattgctgc cggtgctgtg
     4681 ccaagcgcac ggactcaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa
     4741 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggcctcac
     4801 cccagagcag gtcgtggcaa tcgcctccaa cattggcggg aaacaggcac tcgagactgt
     4861 ccagcgcctg cttcccgtgc tgtgccaagc gcacggatta accccagagc aggtcgtggc
     4921 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt
     4981 gctgtgccaa gcgcacggct taaccccaga gcaggtcgtg gccattgcct cgaatggagg
     5041 gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg
     5101 acttacccca gagcaggtcg tggcgatcgc aagccacgac ggaggaaagc aagccttgga
     5161 aacagtacag aggctgttgc ctgtgctgtg ccaagcgcac ggccttaccc cagagcaggt
     5221 cgtggcgatc gcaagccacg acggaggaaa gcaagccttg gaaacagtac agaggctgtt
     5281 gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcga tcgcaagcca
     5341 cgacggagga aagcaagcct tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc
     5401 gcacgggctc accccagagc aggtcgtggc cattgcctcg aatggagggg gcaaacaggc
     5461 gttggaaacc gtacaacgat tgctgccggt gctgtgccaa gcgcacgggc taaccccaga
     5521 gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag gctttggaaa cggtgcagag
     5581 gctccttcca gtgctgtgcc aagcgcacgg cctaacccca gagcaggtcg tggcaatcgc
     5641 ctccaacatt ggcgggaaac aggcactcga gactgtccag cgcctgcttc ccgtgctgtg
     5701 ccaagcgcac gggttaaccc cagagcaggt cgtggcgatc gcaagccacg acggaggaaa
     5761 gcaagccttg gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc acggactcac
     5821 gcctgagcag gtagtggcta ttgcatccaa tatcgggggc agacccgcac tggagtcaat
     5881 cgtggcccag ctttcgaggc cggaccccgc gctggccgca ctcactaatg atcatcttgt
     5941 agcgctggcc tgcctcggcg gacgacccgc cttggatgcg gtgaagaagg ggctcccgca
     6001 cgcgcctgca ttgattaagc ggaccaacag aaggattccc gagaggacat cacatcgagt
     6061 ggcagatcac gcgcaagtgg tccgcgtgct cggattcttc cagtgtcact cccaccccgc
     6121 acaagcgttc gatgacgcca tgactcaatt tggtatgtcg agacacggac tgctgcagct
     6181 ctttcgtaga gtcggtgtca cagaactcga ggcccgctcg ggcacactgc ctcccgcctc
     6241 ccagcggtgg gacaggattc tccaagcgag cggtatgaaa cgcgcgaagc cttcacctac
     6301 gtcaactcag acacctgacc aggcgagcct tcatgcgttc gcagactcgc tggagaggga
     6361 tttggacgcg ccctcgccca tgcatgaagg ggaccaaact cgcgcgtcag ctagccccaa
     6421 gaagaagaga aaggtggagg ccagcatgga cgcgaaatca cttacggcat ggtcgagaac
     6481 actggttacg ttcaaggacg tgtttgtgga ctttacacgt gaggagtgga aattgctgga
     6541 tactgcgcaa caaattgtgt atcgaaatgt catgcttgag aattacaaga acctcgtcag
     6601 tctcggatac cagttgacga aaccggatgt gatccttagg ctcgaaaagg gggaagaacc
     6661 ttggctggta tctagaggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt
     6721 cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6781 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     6841 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6901 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     6961 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7021 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7081 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7141 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     7201 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7261 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7321 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7381 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7441 cgagctgtac aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     7501 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7561 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7621 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7681 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7741 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7801 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7861 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7921 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7981 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8041 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     8101 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     8161 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     8221 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8281 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     8341 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     8401 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     8461 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     8521 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     8581 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8641 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8701 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8761 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     8821 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8881 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8941 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     9001 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     9061 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     9121 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     9181 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9241 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9301 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9361 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     9421 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9481 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     9541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9601 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9661 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9721 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9781 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9841 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     9901 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     9961 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
    10021 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
    10081 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
    10141 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
    10201 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
    10261 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
    10321 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    10381 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    10441 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    10501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10561 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    10621 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10681 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10741 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10801 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10921 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10981 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    11041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11221 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    11281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11341 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11401 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11461 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11521 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11581 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11641 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11701 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11761 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11821 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11881 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11941 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    12001 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    12061 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    12121 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    12181 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    12241 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    12301 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    12361 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    12421 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12481 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12541 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12601 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12661 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12721 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12781 ccacctgac
//
