LOCUS       sequence-58218-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             800..817
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttaaggctag agtacttaat acgactcact ataggctagc ctcgagaatt cacgcgtggt
       61 acctctagag tcgacccggg cggccgcatt ggaagttctg tttcaagggc ctgggagcga
      121 caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt
      181 cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg
      241 cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
      301 cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
      361 tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
      421 caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg
      481 gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa
      541 ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg
      601 ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
      661 cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
      721 cgtcttctca tgctccgtga tgcatgaggg tctgcacaac cactacacgc agaagagcct
      781 ctccctgtct ccgggtaaac accatcacca tcaccatta
//
