LOCUS       sequence-59092-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-Ttag-iresTK
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             197..721
                     /codon_start=1
                     /product="SV40 (simian virus 40) small t antigen"
                     /label=small t antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAIYCKQWPECA
                     KKMSTNCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     intron          443..788
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gctagaggat cccccgggct gcaggaattc
      121 ccggcctctg agctattcca gaagtagtga agaggctttt ttggaggcct aggcttttgc
      181 aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
      241 ccttctaggt cttgaaagga gtgcctgggg gaatattcct ctgatgagaa aggcatattt
      301 aaaaaaatgc aaggagtttc atcctgataa aggaggagat gaagaaaaaa tgaagaaaat
      361 gaatactctg tacaagaaaa tggaagatgg agtaaaatat gctcatcaac ctgactttgg
      421 aggcttctgg gatgcaactg aggtatttgc ttcttcctta aatcctggtg ttgatgcaat
      481 atactgcaaa caatggcctg agtgtgcaaa aaaaatgtct actaactgca tatgcttgct
      541 gtgcttactg aggatgaagc atgaaaatag aaaattatac aggaaagatc cacttgtgtg
      601 ggttgattgc tactgcttcg attgctttag aatgtggttt ggacttgatc tttgtgaagg
      661 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctata
      721 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
      781 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
      841 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
      901 ctcaacattc tactcctcc
//
