LOCUS       sequence-59105-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-Ttag-iresTK
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(440..457)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(734..750)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(735..751)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ccttcaggtc agggaattaa taacctggac aatttaaggg attatttgga tggcagtgtt
       61 aaggtaaact tagaaaagaa acacctaaat aaaagaactc aaatatttcc ccctggaata
      121 gtcaccatga atgagtacag tgtgcctaaa acactgcagg ccagatttgt aaaacaaata
      181 gattttaggc ccaaagatta tttaaagcat tgcctggaac gcagtgagtt tttgttagaa
      241 aagaggataa ttcaaagtgg cattgctttg cttcttatgt taatttggta cagacctgtg
      301 gctgagtttg ctcaaagtat tcagagcaga attgtggagt ggaaagagag attggacaaa
      361 gagtttagtt tgtcagtgta tcaaaaaatg aagtttaatg tggctatggg agttggagtt
      421 ttagattggc taagaaacag tgatgatgat gatgatgaca gccaggaaaa tgctgataaa
      481 aatgaagatg gtggggagaa gaacatggaa gactcagggc atgaaacagg cattgattca
      541 cagtctcaag gctcatttca ggcccctcag ccctcacagt cctcacagtc tgttcatgat
      601 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
      661 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttagcggcc ccggaattcg
      721 atatcaagct tatcgatacc gtcgacctcg acattaattc cggttatttt ccaccatatt
      781 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
      841 taggggtctt tcccctctcg ccaaag
//
