LOCUS       sequence-59108-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ajith Karunarathne WK, O'Neill PR, Martinez-Espinosa PL, 
            Kalyanaraman V, Gautam N
  TITLE     All G protein betagamma complexes are capable of translocation on 
            receptor activation.
  JOURNAL   Biochem Biophys Res Commun. 2012 May 11;421(3):605-11. doi: 
            10.1016/j.bbrc.2012.04.054. Epub 2012 Apr 19.
  PUBMED    22538369
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.04.054"; journalName: "Biochem Biophys Res 
            Commun"; date: "2012-05-11- 11"; volume: "421"; issue: "3"; pages: 
            "605-11"
COMMENT     Sequence Label: Venus-Gprotein Gamma 9
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(27..29,30..32,33..491)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(751..768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    757..868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctgcgtttac ttagcttggt accaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtacccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      481 atatcaccgc cggcggatcc ggtggaggca tggcccaaga gctcagcgag aaggaactct
      541 taaagatgga ggtagagcag ctgaagaagg aagtgaagaa cccaagagct ctgatttcca
      601 aaacaggaaa ggaaatcaag gattatgtgg aggctgaagc aggaaacgac cctcttctta
      661 aaggcatccc tgaggacaag aatcccttca aggaaaaagg cggttgtata ataagctgat
      721 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      901 gggggtgggg ggtgggggca ggacagcagg ggggaaggat tgggtaagac aacatccggc
      961 ttcttgggga tgcgtgtagg tcctatggct gtgtgagagg aggaaagaac agacttggag
     1021 agtttagtcg tttccccagc tagcgctgga tgtgng
//
