LOCUS       sequence-59118-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, 
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, 
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex 
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. 
            Epub 2012 Dec 13.
  PUBMED    23245941
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.026"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1457-73"
COMMENT     Sequence Label: TBX5-FLAG in pLX303
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(136..180)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(193..216)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgcc accggttagc gctagctcat
       61 tactaaacca ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgcctacct
      121 cgagctcggc gcgccttcgt gccattcgat tttctgagcc tcgaagatgt cgttcaggcc
      181 ggacccatca cctttatcgt catcgtcttt gtagtcacgc gtgctattgt cgctccactc
      241 tggcaccatg ccaactccgt gcacagagtg gtactgatga ggggatagag tccttggcac
      301 gccatgagag tagaggaact cagggggctg aagggtgcca ggggactgca ggccagtctg
      361 aggcccacac tgcctgacca caggctggtg ggccacggag gtctggtgct ggaacattcc
      421 ctctcccagc tgtggggagc catggttggc catgccagcc agccgaggga ccaggggccc
      481 cgaggtgaag tgagcggaga agtgctggta gggtagcctg tccatgggct gcacggtggt
      541 gacggtgcag ctgctgtagg aaggcatgct tggccacgtg ttgcagctga tgtcctctag
      601 gctgggcaca ggctcgctgg ggggcgcaga gctggcatac atgcaagctt gccgctgtgc
      661 cgactctgtc ctgtaggagg cacccaggcc ctgctgctgt ggatagctag agcggtagaa
      721 ggaatcttct tcactgggtg atgtctccat gtagggcttc ttatagggat ggtctgtggt
      781 ggaacattct tcctctttcc tcttggtaca atggtaaatt tggctatgct cctggggcag
      841 tgggtatggg
//
