LOCUS       sequence-59120-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human ATM with Flag and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, 
            Appella E, Kastan MB, Siliciano JD
  TITLE     Activation of the ATM kinase by ionizing radiation and 
            phosphorylation of p53.
  JOURNAL   Science. 1998 Sep 11;281(5383):1677-9.
  PUBMED    9733515
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1677-9"
COMMENT     Sequence Label: pcDNA3.1(+)Flag-His-ATM wt
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac
       61 actggcggcc gctcgaccac catggactac aaggacgatg acgacaagat gcatcatcat
      121 catcatcata tgagtctagt acttaatgat ctgcttatct gctgccgtca actagaacat
      181 gatagagcta cagaacgaaa gaaagaagtt gagaaattta agcgcctgat tcgagatcct
      241 gaaacaatta aacatctaga tcggcattca gattccaaac aaggaaaata tttgaattgg
      301 gatgctgttt ttagattttt acagaaatat attcagaaag aaacagaatg tctgagaata
      361 gcaaaaccaa atgtatcagc ctcaacacaa gcctccaggc agaaaaagat gcaggaaatc
      421 agtagtttgg tcaaatactt catcaaatgt gcaaacagaa gagcacctag gctaaaatgt
      481 caagaactct taaattatat catggataca gtgaaagatt catctaatgg tgctatttac
      541 ggagctgatt gtagcaacat actactcaaa gacattcttt ctgtgagaaa atactggtgt
      601 gaaatatctc agcaacagtg gttagaattg ttctctgtgt acttcaggct ctatctgaaa
      661 ccttcacaag atgttcatag agttttagtg gctagaataa ttcatgctgt taccaaagga
      721 tgctgttctc agactgacgg attaaattcc aaatttttgg actttttttc caaggctatt
      781 cagtgtgcga gacaagaaaa gagctcttca ggtctaaatc atatcttagc agctcttact
      841 atcttcctca agactttggc tgtcaacttt cgaattcgag
//
