LOCUS       sequence-59124-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Morriswood B, Havlicek K, Demmel L, Yavuz S, Sealey-Cardona M, 
            Vidilaseris K, Anrather D, Kostan J, Djinovic-Carugo K, Roux K, 
            Warren G
  TITLE     Novel bilobe components in Trypanosoma brucei identified using 
            proximity-dependent biotinylation.
  JOURNAL   Eukaryot Cell. 2012 Dec 21.
  PUBMED    23264645
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell. 2012 Dec 21."
COMMENT     Sequence Label: pLew100_myc_BirA*
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(392..421)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    554..572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    575..593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggttggctcc gaatgggctg aaccacttcc gtcctctgcc tcctctgcca gcctgctggt
       61 actcggcgat gcaggcgtcg ccggacttca gctctccgat tctgtccagc aggtactgat
      121 tggtggagtc gatcacaggc agcacggcca cgcttccgcc atccagctgt cccaggatct
      181 gcttggcgtt cagcagctgg ataggctcag gcaggctgta gcccttgcca ggcacggtga
      241 acacgtccac tccccagtct ctcagtgtct ggatgtgctt gttgatggcg gctctgctca
      301 ttcccagggt ctctcccagc tgctcgccag agtggaactc gccgttggcc agcagggcga
      361 tcagcttcag gggcacggtg ttgtccttgt cgagatcctc ttctgagatg agtttttgtt
      421 ccatatgaag cttgtgaatt ttactttttg gtgtaattga agtcttcaag ttgaaaaaaa
      481 tggggaaaag gaaaaaaaac tcgaaggcgc gtgcagcccg ggacgagaac atgttaaact
      541 cagtactcag ggatctctat cactgatagg gagatctcta tcactgatag ggagatccct
      601 acccgataac ccctattttc aatgcacgcc cacccagaga cacggaatag cataaaacaa
      661 ccccatcact gccacaaaat ggacattttg cacaaaacgc actattgact agtattcgac
      721 tatactaata agagatacct atcagacacg atatactgtg aaaaggacat taagctcact
      781 atgcatctga aaacgctttc acctcacaac ctactgacac acacctttcc gcttaacccc
      841 aatgac
//
