LOCUS       sequence-59149-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of 
            RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p212 pCMV-EGFP/RFP
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    164..285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(201..220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     255..274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(292..325)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(544..564)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg actcagatcc accggatcta gataactgat
       61 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      121 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      181 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      241 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg tataacttcg
      301 tatagcatac attatacgaa gttatcggta ccgcgggccc gggatccacc ggtcgccacc
      361 atggtgcgct cctccaagaa cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
      421 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      481 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      541 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
      601 cccgactaca
//
