LOCUS       sequence-59160-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Brtva TR, Drugan JK, Ghosh S, Terrell RS, Campbell-Burk S, Bell RM, 
            Der CJ
  TITLE     Two distinct Raf domains mediate interaction with Ras.
  JOURNAL   J Biol Chem. 1995 Apr 28. 270(17):9809-12.
  PUBMED    7730360
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Apr 28. 270(17):9809-12."
COMMENT     Sequence Label: Raf-1 GST RBD 1-149
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(554..576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcctccaaa atcggatctg gttccgcgtg gatccccgat ccatattatg gagcacatac
       61 agggagcttg gaagacgatc agcaatggtt ttggattcaa agatgccgtg tttgatggct
      121 ccagctgcat ctctcctaca atagttcagc agtttggcta tcagcgccgg gcatcagatg
      181 atggcaaact cacagatcct tctaagacaa gcaacactat ccgtgttttc ttgccgaaca
      241 agcaaagaac agtggtcaat gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag
      301 cactcaaggt gaggggcctg caaccagagt gctgtgcagt gttcagactt ctccacgaac
      361 acaaaggtaa aaaagcacgc ttagattgga atactgatgc tgcgtctttg attggagaag
      421 aacttcaagt agatttcctg gatcatgttc ccctcacaac acacaacttt gctcggaaga
      481 cgttcctgaa gctgggaatt catcgtgact gactgacgat ctgcctcgcg cgtttcggtg
      541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
      601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      661 gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattccctt
//
