LOCUS       sequence-59177-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dead subunit for creating monovalent streptavidin with a single 
            femtomolar biotin binding site. Use in combination with 
            Streptavidin-Alive (construct 20860)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Howarth M, Chinnapen DJ, Gerrow K, Dorrestein PC, Grandy MR, 
            Kelleher NL, El-Husseini A, Ting AY
  TITLE     A monovalent streptavidin with a single femtomolar biotin binding 
            site.
  JOURNAL   Nat Methods. 2006 Apr . 3(4):267-73.
  PUBMED    16554831
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Apr . 3(4):267-73."
COMMENT     Sequence Label: pET21a-Streptavidin-Dead
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(531..549)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      545..592
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(716..735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     926..947
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tggctgaagc
       61 tggtatcacc ggcacctggt acgcccagct gggagacacc ttcatcgtta ccgctggtgc
      121 tgacggtgct ctgaccggta cctacgaagc cgctgttggt aacgctgaat ctagatacgt
      181 tctgaccggt cgttacgact ccgctccggc taccgacggt tccggaaccg ctctgggttg
      241 gaccgttgct tggaaaaaca actaccgtaa cgctcactcc gctaccacct ggtctggcca
      301 gtacgttggt ggtgctgaag ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac
      361 cgaagctaac gcgtggaaat ccaccctggt tggtcacgac accttcacca aagttaaacc
      421 gtccgctgct tcctaataaa agcttgcggc cgcactcgag caccaccacc accaccactg
      481 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      601 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      661 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      721 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      781 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      841 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      901 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      961 cctat
//
