LOCUS       sequence-59249-e       11297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: FU-MAP2-Gateway
FEATURES             Location/Qualifiers
     source          1..11297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     4091..4110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4091..4109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4141..4265
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4290..4295,4296..4313,4314..4320)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4374..5033
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4440..4459)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5375..5680
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5559..5578
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5721..5845)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5871..5912
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5989..6577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6042..6062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6460..6471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6580..6596)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6581..6597)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7102..7282
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7308..7325)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7314..7538
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7584..8012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7671..7690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7881..7902
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8021..8041)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8026..8355
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8206..8341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8268..8287
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8403..8450
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8469..8843
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8973..9094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9010..9029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9064..9083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9143..9159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9143..9159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9156..9178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9167..9183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9191..9197,9198..9215,9216..9221))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9236..9257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9374..9391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9545..10133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9625..9644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10304..11095,11096..11164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10927..10946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11165..11269)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acagctggcc tttttggttc tcatcgaatt ttgctccatt
     2641 cactggttca ggttgctaac aatgctgtaa aaattaactt atttattgac atttttctcc
     2701 ttcccttttc cccaggaaat aaatgcagga aattattcca cacttaacct ccctacacaa
     2761 gggcactgta aaatagggat attcaactgc taatttcagt tgctaacatg gaggggatac
     2821 agattatgcc aaagattgaa ttctctgcct gggccctcag ggactgaaaa gccccaggac
     2881 caagctggtt gccacctcct tttgcttttg tatgtaaata gattgccccc agaaatcaca
     2941 tttctaattc tctgtttttt ttttcttttt ctcttgtaag gaaaacatgg ccaagtgcat
     3001 aattataact gttaaaaggg tattgaattt tctgtaggaa aatatacata aatatatgca
     3061 tttctttaat gaagatattt attttagaat ataaataagc aatatgcgat agttaaaaat
     3121 attgtaatta tctgccaatg acaaaacatc aacaaatctc tgatgtcctt aatgcagata
     3181 ataaaaattc ccataaacta gtatttagtc atgtccagtt tgtgtagctt aacttgtttg
     3241 taatcctata tgcctgaaaa ctgtttctct atttaagcgg tgtgtgtgtg tgcgtgcgtg
     3301 cgtgcgtgtg tgtgtgtgtg tgtctggcat taacacaaag gagaccagat gatgacattc
     3361 ataggctgat agactcgact cctggggaca cacttggatg gaggcaatta aaatgcctgt
     3421 caggcatttg gtaccagaag cccctatggc aaaccgctaa atcgtaagtg agggctgcat
     3481 cctattgtca gtgaatgatg gcggagctga gtggcttgtc atcattctcg aaaattctga
     3541 gactagtgag ctctctccct ctctcccact cgccttattt tcctgttcgc cactgcgggg
     3601 acttctaatt tgcagcttcc ctttccccgg cacacacaga cacgagctgg tggcttgcag
     3661 actgcgtctg tctggagcta gagagccaga gagccagcct gtggggataa tgctcccgga
     3721 gaaggattct gcagcagttc tcaaaggcta gacttgagtg gtattgctgc atatgcgctg
     3781 gtaatggttt ggggctttta attttatttc gtttcatttt caattcgttt ttcataggat
     3841 gtgctttatg taatctcaga gtaaagatct tttaaaattc attattgagc ttcctaaata
     3901 ttcaagtact gcattttttt tgtgctacag acctccaggc atagatttat tatttgagta
     3961 taaagaatgc atttgcttac tggtgagtta taatttttga tatttagtta acaaatccat
     4021 atatatatat atatatgttt cttttgtttt gtttcggtag attcttcagc ttgtctctaa
     4081 ccgactcgag taatacgact cactataggg agacccaagc tggctaggta agcttgatca
     4141 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     4201 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     4261 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     4321 tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga
     4381 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     4441 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     4501 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     4561 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     4621 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     4681 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     4741 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     4801 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     4861 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     4921 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     4981 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     5041 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     5101 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     5161 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     5221 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     5281 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     5341 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     5401 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     5461 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     5521 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     5581 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     5641 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     5701 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     5761 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     5821 gttcagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ggtaagccta
     5881 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcgc catcaccatt
     5941 gagtttaaac ccgctgatca gcctcaattc gatatcaagc ttatcgataa tcaacctctg
     6001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6361 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6541 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     6601 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6661 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6721 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6781 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6841 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6901 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     6961 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7021 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     7081 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7141 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7201 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7261 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7321 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7381 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7441 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7501 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7561 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7921 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7981 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     8041 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8101 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8161 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8221 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8281 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8341 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     8401 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     8461 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     8521 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     8581 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     8641 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     8701 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     8761 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     8821 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     8881 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8941 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     9001 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9061 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     9121 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9361 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9421 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9481 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9601 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9721 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9961 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    10021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    10081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11281 gaaaagtgcc acctgac
//
