LOCUS       sequence-59252-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH039
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(66..86)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccctcagcgc ggccgcggta ccctatttgt atagttcatc catgccatgt gtaatcccag
       61 cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa
      121 gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg
      181 gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa
      241 ttttgaagtt ctgaaaattt aaatcagatt agttagttag taaaatcatg tttaaactta
      301 ccgctttgat cccattcttt tgtttgtcgg cagtgatgta tacgttgtgt gaaatatagt
      361 tgtattccaa tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact
      421 cgattctatt aacaagggta tcaccttcaa acttgacttc agcacctgaa aatttaaata
      481 tgtatggtta gttagtaccg aactgtttaa acttacgtgt cttgtagttc ccgtcatctt
      541 tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      601 gctgtttcat atgatctggg tatctcgaga agcattgaac gccccatgag aaagtagtga
      661 caagtgttgg ctgaaaattt aaataatcag ggttagttag tatatatatg tttaaactta
      721 cccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg
      781 ttgcatcacc ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta
      841 attcaaca
//
