LOCUS       sequence-59255-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD6
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..42)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(43..78)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             complement(88..111)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(165..183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(226..245)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          228..360
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(475..499)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(496..699)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(525..545)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atccagaggt tgattctagt gatggtgatg gtgatggtga tgcttgccat caatcaatct
       61 tggatcaact tgatcttctg cggccccagg cccttgaaac agaacttcca atgcggccgc
      121 ccgggtcgac tctagaggta ccacgcgtga attctcgagg ctagcctata gtgagtcgta
      181 ttaagtactc tagccttaag agctgtaatt gaactgggag tggacacctg tggagagaaa
      241 ggcaaagtgg atgtcagtaa gaccaatagg tgcctatcag aaacgcaaga gtcttctctg
      301 tctcgacaag cccagtttct attggtctcc ttaaacctgt cttgtaacct tgatacttac
      361 ctgcccagtg cctcacgacc aacttctgca gcttaagttc gagactgttg tgtcagaagc
      421 actgactgcg ttagcaattt aactgtgata aactaccgca ataaagcttc tagtgatctg
      481 acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      541 ttgcgtcaac ggggcggggt tattacgaca ttttggaaag tcccgttgat tttggtgcca
      601 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      661 ctatccacgc ccattggtgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
      721 tacgtagatg tactgccaag taggaaagtc ccgtaaggtc atgtactggg cataatgcca
      781 ggcgggccat ttaccgtcat tgacgtcaat agggggcgga
//
