LOCUS       sequence-59256-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD6L
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(49..84)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             complement(94..117)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(239..257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(300..319)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          302..434
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(549..573)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(570..773)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(599..619)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tttgtaatcc agaggttgat tctagtgatg gtgatggtga tggtgatgct tgccatcaat
       61 caatcttgga tcaacttgat cttctgcggc cccaggccct tgaaacagaa cttccaatgc
      121 ggccgcccgg gtcgactcta gaggtaccac gcgtgaattc gccaggaaac cagagcaaga
      181 gcaaccccag caattgagca ggaactctca tatccatggt ggcaattctc gaggctagcc
      241 tatagtgagt cgtattaagt actctagcct taagagctgt aattgaactg ggagtggaca
      301 cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc
      361 aagagtcttc tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta
      421 accttgatac ttacctgccc agtgcctcac gaccaacttc tgcagcttaa gttcgagact
      481 gttgtgtcag aagcactgac tgcgttagca atttaactgt gataaactac cgcaataaag
      541 cttctagtga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca
      601 cgcctaccgc ccatttgcgt caacggggcg gggttattac gacattttgg aaagtcccgt
      661 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      721 cgtgagtcaa accgctatcc acgcccattg gtgtactgcc aaaaccgcat caccatggta
      781 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccgtaa ggtca
//
