LOCUS       sequence-59258-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(731..754)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtaatc cagaggttga ttctaatggt gatggtgatg gtgtttaccc ggagacaggg
       61 agaggctctt ctgcgtgtag tggttgtgca gaccctcatg catcacggag catgagaaga
      121 cgttcccctg ctgccacctg ctcttgtcca cggtgagctt gctgtagagg aagaaggagc
      181 cgtcggagtc cagcacggga ggcgtggtct tgtagttgtt ctccggctgc ccattgctct
      241 cccactccac ggcgatgtcg ctgggataga agcctttgac caggcaggtc aggctgacct
      301 ggttcttggt catctcctcc cgggatgggg gcagggtgta cacctgtggt tctcggggct
      361 gccctttggc tttggagatg gttttctcga tgggggctgg gagggctttg ttggagacct
      421 tgcacttgta ctccttgcca ttcagccagt cctggtgcag gacggtgagg acgctgacca
      481 cacggtacgt gctgttgtac tgctcctccc gcggctttgt cttggcatta tgcacctcca
      541 cgccgtccac gtaccagttg aacttgacct cagggtcttc gtggctcacg tccaccacca
      601 cgcatgtgac ctcaggggtc cgggagatca tgagggtgtc cttgggtttt ggggggaaga
      661 ggaagactga cggtcccccc aggagttcag gtgctgggca cggtgggcat gtgtgagttt
      721 tgtcgctccc aggcccttga aacagaactt ccaatgcggc cgcccgggtc gactctagag
      781 gtacca
//
