LOCUS       sequence-59261-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8L
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(719..742)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gaggttgatt ctaatggtga tggtgatggt gtttacccgg agacagggag aggctcttct
       61 gcgtgtagtg gttgtgcaga ccctcatgca tcacggagca tgagaagacg ttcccctgct
      121 gccacctgct cttgtccacg gtgagcttgc tgtagaggaa gaaggagccg tcggagtcca
      181 gcacgggagg cgtggtcttg tagttgttct ccggctgccc attgctctcc cactccacgg
      241 cgatgtcgct gggatagaag cctttgacca ggcaggtcag gctgacctgg ttcttggtca
      301 tctcctcccg ggatgggggc agggtgtaca cctgtggttc tcggggctgc cctttggctt
      361 tggagatggt tttctcgatg ggggctggga gggctttgtt ggagaccttg cacttgtact
      421 ccttgccatt cagccagtcc tggtgcagga cggtgaggac gctgaccaca cggtacgtgc
      481 tgttgtactg ctcctcccgc ggctttgtct tggcattatg cacctccacg ccgtccacgt
      541 accagttgaa cttgacctca gggtcttcgt ggctcacgtc caccaccacg catgtgacct
      601 caggggtccg ggagatcatg agggtgtcct tgggttttgg ggggaagagg aagactgacg
      661 gtccccccag gagttcaggt gctgggcacg gtgggcatgt gtgagttttg tcgctcccag
      721 gcccttgaaa cagaacttcc aatgcggccg cccgggtcga ctctagaggt acc
//
