LOCUS       sequence-59263-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Tryon RC, Pisat N, Johnson SL, Dougherty JD
  TITLE     Development of translating ribosome affinity purification for 
            zebrafish.
  JOURNAL   Genesis. 2012 Dec 19. doi: 10.1002/dvg.22363.
  PUBMED    23281262
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2012 Dec 19. doi: 10.1002/dvg.22363."
COMMENT     Sequence Label: Tol2-zTRAP
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             75..83
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    698..832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(722..741)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     776..795
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tccggccgga ctcagatctc gagctcaagc ttcgaattca gcaaggtctc gagagacacg
       61 ttgtacgagg ccgttaagga ggtccaggcc ggttcccgtc gcaagaagag aaagtttctt
      121 gaaaccgtgg aactccagat cagcttgaag aactacgacc ctcagaagga caagcgtttc
      181 tcaggcactg tcaggctgaa gaccaccccg cggcccaagt tctctgtctg cgttcttgga
      241 gaccagcagc attgtgatga agcaaaggcc gctgaactgc cacacatgga cattgaggct
      301 cttaagaagc tcaataagaa caagaaactg gtcaagaagc tggcaaagaa gtacgatgct
      361 ttcctggcct ctgagtctct gatcaagcag attcctcgta tcctgggccc tggcctcaac
      421 aaggcaggaa agtttccctc tctgctcacc cataatgaga acctgggcac caaggtggat
      481 gaggtgaaat ccaccatcaa attccagatg aagaaggttc tgtgtctggc tgtggccgtg
      541 ggtcatgtga agatgagtga ggaagagctg gtctacaaca tccacctggc tgtcaacttc
      601 ctggtgtctc tgctgaagaa gaactggcaa aacgtcagag ctctttacat caagagcacc
      661 atgggaaaac cacagcgcct ctactaggat cgatgatgat ccagacatga taagatacat
      721 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      781 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag tta
//
