LOCUS       sequence-59267-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     inducible Drosophila S2 vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCGMbX
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    140..274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(177..196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     231..250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     307..324
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(347..364)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(374..415)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     sig_peptide     complement(514..567)
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
ORIGIN
        1 attacgccaa gcttgcatgc ctgcaggtcg actgatcata atcagccata ccacatttgt
       61 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      121 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      241 caaactcatc aatgtatctt atcatgtctg gatcctcgat cgaggctgat cagcgagctc
      301 aggccttaga aggcacagtc gaggctgatc agcgggttta aactcaatgg tgatggtgat
      361 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
      421 agggccctct agactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac
      481 tggactagta ggtaccccgg gccatggaga tctcccgagc gagaggccaa caaaggccac
      541 gacggccagt aatatgcata acttcatatt gagatcggat ccccccttta gttgcactga
      601 gatgattcac ttgattttgc tgctgaccac aactgatgca cattggccag atgctctcgg
      661 ttttatacat tttggaaacg cggctttaca cacgggtctt tcgttcgatt cacctctttt
      721 gcacacgccg gccggctcca aattcgagtg gagacgtgtg cagaagcttt tgttctgccc
      781 gcgaagaatt ggttcggttc tccactttgg ggacttgtat gggatgcaaa gctcttggtg
      841 gggcacatgg ggcatgcgca ggcactctta cttgcgtacc acatatgtat gggggtggcg
      901 gtgggcggtg gggggccgga attctgggtc tcttatcg
//
