LOCUS       sequence-59272-e       10623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV
  TITLE     Optogenetic protein clustering and signaling activation in mammalian
            cells.
  JOURNAL   Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360.
  PUBMED    23377377
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360."
COMMENT     Sequence Label: CLPIT Cry2PHR-mCh-Rac1
FEATURES             Location/Qualifiers
     source          1..10623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..43)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     enhancer        151..454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    901..1258
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1323..1739
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1669..1691
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1707..1723
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2061..2077
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2137..2736
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(2137..2156)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2633..2653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2816..3388,3389..3391,3392..3400)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2982..2999)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3209..3228
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3401..4024,4025..4411)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGST
                     IEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAH
                     ADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3477..3496)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    join(4470..4488,4489..4511,4512..4530,4531..4553,4554..4572,
                     4573..4595,4596..4614,4615..4637,4638..4656,4657..4679,
                     4680..4698,4699..4718,4719..4737)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4770..4807
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4804..4828
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4897..6390
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      6409..6418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6415..7119
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(6560..6578)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6828..6847
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7153..7728
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(7738..7754)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7738..7754)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             8035..8504
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8697..8714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8868..9456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8948..8967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9627..10418,10419..10487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10250..10269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10488..10592)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
       61 tccccagcag gcagaagtat gcaaagtgac tagttattaa tagtaatcaa ttacggggtc
      121 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      181 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      241 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      301 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      361 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      421 gtacatctac gtattagtca tcgctattac catgcatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg
      721 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
      781 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
      841 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
      901 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
      961 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
     1021 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg
     1081 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc
     1141 ccttagagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
     1201 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca
     1261 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc
     1321 cgggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     1381 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     1441 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     1501 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     1561 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     1621 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     1681 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc
     1741 ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc
     1801 ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag gctctctact
     1861 tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac
     1921 cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta
     1981 agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc
     2041 tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg
     2101 ggggtggacc atcctctagc gtttctgaag cttaccatga ccgagtacaa gcccacggtg
     2161 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     2221 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     2281 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     2341 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     2401 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     2461 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     2521 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     2581 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     2641 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc
     2701 gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     2761 ccgaccgaaa ggagcgcacg accccatgca tcgataagct tgatatcgaa ttccgcccct
     2821 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2941 ctggcctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3001 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3061 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3121 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3181 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3241 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3301 ctttacgtgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     3361 gttttccttt gaaaaacacg atgataatat ggccacaacc atgggttcta gattagataa
     3421 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     3481 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     3541 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     3601 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     3661 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     3721 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     3781 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     3841 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     3901 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     3961 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     4021 gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg agggcctgct
     4081 cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc gcctgtcctt
     4141 tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg tcagcctggg
     4201 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     4261 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     4321 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     4381 tgcccttgga attgacgagt acggtgggta gggggcgcga ggatcggaat tgaatatcga
     4441 atgcagcagc aattcgtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4501 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc agtttacact cctatcagtg atagagaaaa
     4741 gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag
     4801 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccaacgc tgttttgcct
     4861 ccaaaagaca ccgggaccga ggccgcctcg gccatgatga agatggacaa aaagactata
     4921 gtttggttta gaagagacct aaggattgag gataatcctg cattagcagc agctgctcac
     4981 gaaggatctg tttttcctgt cttcatttgg tgtcctgaag aagaaggaca gttttatcct
     5041 ggaagagctt caagatggtg gatgaaacaa tcacttgctc acttatctca atccttgaag
     5101 gctcttggat ctgacctcac tttaatcaaa acccacaaca cgatttcagc gatcttggat
     5161 tgtatccgcg ttaccggtgc tacaaaagtc gtctttaacc acctctatga tcctgtttcg
     5221 ttagttcggg accataccgt aaaggagaag ctggtggaac gtgggatctc tgtgcaaagc
     5281 tacaatggag atctattgta tgaaccgtgg gagatatact gcgaaaaggg caaacctttt
     5341 acgagtttca attcttactg gaagaaatgc ttagatatgt cgattgaatc cgttatgctt
     5401 cctcctcctt ggcggttgat gccaataact gcagcggctg aagcgatttg ggcgtgttcg
     5461 attgaagaac tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt gttaactaga
     5521 gcttggtctc caggatggag caatgctgat aagttactaa atgagttcat cgagaagcag
     5581 ttgatagatt atgcaaagaa cagcaagaaa gttgttggga attctacttc actactttct
     5641 ccgtatctcc atttcgggga aataagcgtc agacacgttt tccagtgtgc ccggatgaaa
     5701 caaattatat gggcaagaga taagaacagt gaaggagaag aaagtgcaga tctttttctt
     5761 aggggaatcg gtttaagaga gtattctcgg tatatatgtt tcaacttccc gtttactcac
     5821 gagcaatcgt tgttgagtca tcttcggttt ttcccttggg atgctgatgt tgataagttc
     5881 aaggcctgga gacaaggcag gaccggttat ccgttggtgg atgccggaat gagagagctt
     5941 tgggctaccg gatggatgca taacagaata agagtgattg tttcaagctt tgctgtgaag
     6001 tttcttctcc ttccatggaa atggggaatg aagtatttct gggatacact tttggatgct
     6061 gatttggaat gtgacatcct tggctggcag tatatctctg ggagtatccc cgatggccac
     6121 gagcttgatc gcttggacaa tcccgcgtta caaggcgcca aatatgaccc agaaggtgag
     6181 tacataaggc aatggcttcc cgagcttgcg agattgccaa ctgaatggat ccatcatcca
     6241 tgggacgctc ctttaaccgt actcaaagct tctggtgtgg aactcggaac aaactatgcg
     6301 aaacccattg tagacatcga cacagctcgt gagctactag ctaaagctat ttcaagaacc
     6361 cgtgaagcac agatcatgat cggagcagca gcccgggatc caccggtcgc caccatggtg
     6421 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     6481 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     6541 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     6601 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     6661 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     6721 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     6781 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     6841 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     6901 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     6961 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     7021 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     7081 gagggccgcc actccaccgg cggcatggac gagctgtacg agggtggagg aggtagtgga
     7141 ggaggaggat cacaggccat caagtgtgtg gtggtgggag acggagctgt aggtaaaact
     7201 tgcctactga tcagttacac aaccaatgca tttcctggag aatatatccc tactgtcttt
     7261 gacaattatt ctgccaatgt tatggtagat ggaaaaccgg tgaatctggg cttatgggat
     7321 acagctggac aagaagatta tgacagatta cgccccctat cctatccgca aacagatgtg
     7381 ttcttaattt gcttttccct tgtgagtcct gcatcatttg aaaatgtccg tgcaaagtgg
     7441 tatcctgagg tgcggcacca ctgtcccaac actcccatca tcctagtggg aactaaactt
     7501 gatcttaggg atgataaaga cacgatcgag aaactgaagg agaagaagct gactcccatc
     7561 acctatccgc agggtctagc catggctaag gagattggtg ctgtaaaata cctggagtgc
     7621 tcggcgctca cacagcgagg cctcaagaca gtgtttgacg aagcgatccg agcagtcctc
     7681 tgcccgcctc ccgtgaagaa gaggaagaga aaatgcctgc tgttgtaagt ttaaacggat
     7741 ccactagttc tagaggccgc ggccgcggat ccggattagt ccaatttgtt aaagacagga
     7801 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     7861 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     7921 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     7981 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     8041 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     8101 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     8161 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     8221 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     8281 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     8341 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     8401 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     8461 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacacatg cagcatgtat
     8521 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag tacttaaagt
     8581 tacattggct tccttgaaat aaacatggag tattcagaat gtgtcataaa tctgcattaa
     8641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9061 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9301 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9661 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10621 gtc
//
