LOCUS       sequence-59278-e       10701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV
  TITLE     Optogenetic protein clustering and signaling activation in mammalian
            cells.
  JOURNAL   Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360.
  PUBMED    23377377
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360."
COMMENT     Sequence Label: CLPIT Cry2PHR-mCh-LRP6c
FEATURES             Location/Qualifiers
     source          1..10701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..43)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     enhancer        151..454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    901..1258
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1323..1739
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1669..1691
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1707..1723
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2061..2077
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2137..2736
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(2137..2156)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2633..2653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2816..3388,3389..3391,3392..3400)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2982..2999)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3209..3228
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3401..4024,4025..4411)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGST
                     IEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAH
                     ADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3477..3496)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    join(4470..4488,4489..4511,4512..4530,4531..4553,4554..4572,
                     4573..4595,4596..4614,4615..4637,4638..4656,4657..4679,
                     4680..4698,4699..4718,4719..4737)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4770..4807
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4804..4828
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4894..6387
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      6406..6415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6412..7116
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(6557..6575)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6825..6844
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7816..7832)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7816..7832)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             8113..8582
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8775..8792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8946..9534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9026..9045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9705..10496,10497..10565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10328..10347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10566..10670)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
       61 tccccagcag gcagaagtat gcaaagtgac tagttattaa tagtaatcaa ttacggggtc
      121 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      181 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      241 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      301 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      361 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      421 gtacatctac gtattagtca tcgctattac catgcatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg
      721 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
      781 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
      841 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
      901 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
      961 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
     1021 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg
     1081 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc
     1141 ccttagagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
     1201 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca
     1261 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc
     1321 cgggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     1381 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     1441 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     1501 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     1561 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     1621 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     1681 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc
     1741 ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc
     1801 ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag gctctctact
     1861 tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac
     1921 cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta
     1981 agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc
     2041 tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg
     2101 ggggtggacc atcctctagc gtttctgaag cttaccatga ccgagtacaa gcccacggtg
     2161 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     2221 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     2281 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     2341 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     2401 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     2461 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     2521 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     2581 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     2641 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc
     2701 gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     2761 ccgaccgaaa ggagcgcacg accccatgca tcgataagct tgatatcgaa ttccgcccct
     2821 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2941 ctggcctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3001 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3061 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3121 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3181 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3241 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3301 ctttacgtgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     3361 gttttccttt gaaaaacacg atgataatat ggccacaacc atgggttcta gattagataa
     3421 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     3481 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     3541 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     3601 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     3661 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     3721 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     3781 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     3841 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     3901 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     3961 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     4021 gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg agggcctgct
     4081 cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc gcctgtcctt
     4141 tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg tcagcctggg
     4201 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga
     4261 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     4321 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     4381 tgcccttgga attgacgagt acggtgggta gggggcgcga ggatcggaat tgaatatcga
     4441 atgcagcagc aattcgtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4501 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc agtttacact cctatcagtg atagagaaaa
     4741 gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag
     4801 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccaacgc tgttttgcct
     4861 ccaaaagaca ccgggaccga ggccgcctcg gccatgaaga tggacaaaaa gactatagtt
     4921 tggtttagaa gagacctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa
     4981 ggatctgttt ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga
     5041 agagcttcaa gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct
     5101 cttggatctg acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt
     5161 atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta
     5221 gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac
     5281 aatggagatc tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg
     5341 agtttcaatt cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct
     5401 cctccttggc ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt
     5461 gaagaactag ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct
     5521 tggtctccag gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg
     5581 atagattatg caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg
     5641 tatctccatt tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa
     5701 attatatggg caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg
     5761 ggaatcggtt taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag
     5821 caatcgttgt tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag
     5881 gcctggagac aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg
     5941 gctaccggat ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt
     6001 cttctccttc catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat
     6061 ttggaatgtg acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag
     6121 cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac
     6181 ataaggcaat ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg
     6241 gacgctcctt taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa
     6301 cccattgtag acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt
     6361 gaagcacaga tcatgatcgg agcagcagcc cgggatccac cggtcgccac catggtgagc
     6421 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     6481 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     6541 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     6601 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     6661 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     6721 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     6781 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     6841 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     6901 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     6961 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     7021 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     7081 ggccgccact ccaccggcgg catggacgag ctgtacgatg gtggaggagg tagtggagga
     7141 ggaggatcaa ggatgttgtg tccacgtatg aagggagatg gggaaactat gactaatgac
     7201 tatgtagttc atggaccagc ttctgtgcct cttggttatg tgccacaccc aagttctttg
     7261 tcaggatctc ttccaggaat gtctcgaggt aaatcaatga tcagctccct cagtatcatg
     7321 gggggaagca gtggaccccc ctatgaccga gcccatgtta caggagcatc atcaagtagt
     7381 tcttcaagca ccaaaggcac ttacttccct gcaattttga accctccacc atccccagcc
     7441 acagagcgat cacattacac tatggaattt ggatattctt caaacagtcc ttccactcat
     7501 aggtcataca gctacaggcc atatagctac cggcactttg caccccccac cacaccctgc
     7561 agcacagatg tttgtgacag tgactatgct cctagtcgga gaatgacctc agtggcaaca
     7621 gccaagggct ataccagtga cttgaactat gattcagaac ctgtgccccc acctcccaca
     7681 ccccgaagcc aatacttgtc agcagaggag aactatgaaa gctgcccacc ttctccatac
     7741 acagagagga gctattctca tcacctctac ccaccgccac cctctccctg tacagactcc
     7801 tcctgagttt aaacggatcc actagttcta gaggccgcgg ccgcggatcc ggattagtcc
     7861 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     7921 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     7981 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     8041 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     8101 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     8161 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     8221 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     8281 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     8341 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     8401 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     8461 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     8521 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     8581 cacacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     8641 ggccatagta cttaaagtta cattggcttc cttgaaataa acatggagta ttcagaatgt
     8701 gtcataaatc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8761 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8821 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8881 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8941 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9001 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9061 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9121 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9181 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9241 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9301 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9361 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9421 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9481 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9541 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9601 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9661 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9721 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9781 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9841 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9901 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9961 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10021 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10081 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10141 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10201 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10261 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10321 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10381 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10441 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10501 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10561 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10621 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10681 cgaaaagtgc cacctgacgt c
//
