LOCUS       sequence-59281-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL027A
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ttatttatac agttcatcca
       61 taccatgcgt aatgccggcc gcggtcacaa attccagcag aaccatgtga tcgcgttttt
      121 cattcggatc tttagacagt ttgctctggt aactcagata atgattatcc ggcagcagca
      181 ccggaccatc gccaatcggg gtgttctgct ggtaatgatc ggccagctga acaccgccat
      241 cttcgatgtt gtgacggatt ttgaagtttg ctttgatacc gtttttctgt ttatcggcgg
      301 taatgtacac gttatgactg ttgtagttgt attccagttt gtgacccaga atattgccat
      361 cttctttgaa atcgatacct ttcagttcaa tacggttcac cagcgtatcg ccttcaaatt
      421 taacttccgc gcgggttttg tagttaccat catctttgaa aaagatggta cgttcctgca
      481 catagccttc cggcatcgca gatttaaaga aatcatggcg tttcatgtga tccgggtaac
      541 gtgcgaaaca catcagacca taacccagcg tggtcaccag ggtcggccac ggaaccggca
      601 gtttacccgt ggtgcaaatc agtttcagcg tcagtttgcc gtaggtcgca tcaccttcgc
      661 cttcaccaga cacgctaaat ttatgaccgt tcacatcgcc atccagttca accaggatcg
      721 gaaccacacc cgtgaacagt tcttcgcctt tgctcataga ttattctcct tataagttca
      781 aactaattga attcggatcc gcttatttat acagctca
//
